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Basic Plant Pathology Methods, Second Edition

Onkar D. Dhingra, Ph.D., Departmento de Fitopatologia, Universidade Federal de Vicosa, Minas Gerais, Brazil

James B. Sinclair, Ph.D., Department of Plant Pathology, University of Illinois at Urbana-Champaign

List Price: $154.95 Our Price: $144.95 Cat. #: L638 ISBN: 0873716388 Publication Date: 4/5/1995 Number of Pages: 448 Availability: In Stock CRC Press


The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations.


The Second Edition includes chapters on the following:

  • Sterilization of culture apparatus and culture media
  • Culture of pathogens with detailed techniques for 61 fungi and selected bacteria
  • Long-term storage of plant pathogens
  • Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera
  • - 15 methods for airborne inoculum and 13 methods for seedborne pathogens
  • Establishment of disease and testing for disease resistance
  • Work with soil microorganisms
  • Fungicide evaluation
  • Biological control
  • Bright-field microscopy


Plant pathologists, microbiologists, and mycologists in both classroom and laboratory, as well as students taking courses in plant pathology methods, introduction to plant pathology, and plant disease control methods.


  • Sterilization of Apparatus and Culture Media
  • Heat Sterilization
  • Gas Sterilization
  • Filtration
  • Culture of Pathogens
  • Factors Affecting the Cultivation and Sporulation of Pathogenic Fungi
  • Some Special Methods of Inducing Sporulation in Fungi
  • Cultivation of Pathogens for Soil Infestation
  • Other Sources of Inoculum
  • Cultivation and Sporulation of Some Pathogens
  • Long-Term Storage of Plant Pathogens
  • Periodic Transfer
  • Mineral Oil
  • Drying
  • Storage in Water
  • Storage in Silica Gel
  • Storage in Soil
  • Freezing
  • Preservation in Liquid Nitrogen
  • Lyophilization and Vacuum Drying
  • Detection and Estimation of Inoculum
  • Soilborne Pathogens
  • Detection and Estimation of Inoculum in the Air
  • Detection of Pathogens Associated with Seeds
  • Establishment of Disease and Testing for Resistance
  • Factors Influencing Disease Establishment
  • Root Inoculation
  • Stem Inoculation
  • Leaf Inoculation
  • Inoculation of Cereal Inflorescences
  • Seed Inoculation
  • Inoculation with Bacteria
  • Inoculation of Detached Plant Parts
  • Field Inoculation Methods
  • Some Special Hints
  • Soil Microorganisms
  • Isolation and Enumeration of Soil Organisms
  • Observation of Soil Microorganisms In Situ
  • Infrared Photography
  • Physiological Processes as an Estimate of Biological Activity in Soil
  • Spore Germination in Soil and Soil Fungistasis
  • Survival of Plant Pathogens in Soil
  • Mycelial Growth in Soil
  • Collection of Root and Seed Exudates
  • Artificial Rhizosphere or Spermosphere
  • Fungicide Evaluation
  • Spore Germination Test
  • Tests Based on Growth on Organisms In Vitro
  • Transfer-Bioassay Techniques
  • Laboratory Testing for Soil Fungicides
  • Greenhouse Testing of Foliar Fungicides
  • Greenhouse Testing of Fungicides for Control of Soilborne Pathogens
  • Planning Field Experiments
  • Seed Treatment with Chemicals
  • Biological Control
  • Collection and Testing of Soil Samples for Antagonists
  • Isolation of Antibiotic-Producing Organisms
  • Isolation and Testing of Lytic Organisms
  • Isolation and Testing of Mycoparasitic Organisms
  • Spore Perforating Amoebae
  • Isolation of Antagonists from Leaves
  • Testing Antagonists for Biological Control
  • Bright-Field Microscopy Techniques
  • Mounting and Staining of Fungi for Microscopic Examination
  • Staining of Fungal Nucelli
  • Mounting Spores for Viewing from Different Positions
  • Immobilization of Fungus Spores in Water Mounts for Microscopic Examination
  • Staining of Bacteria
  • Cements for Sealing Mounts
  • Slide Culture Techniques and Preparation of Permanent Mounts
  • Preparing Agar-Colony Mounts and Dry Reference Cultures
  • Examination of Host-Parasite Relationship Using Bright-Field Microscopy
  • Appendices
  • Culture Media and Their Formulas
  • Plant Nutrient Solutions
  • Solutions to Maintain Constant Humidity in a Closed Atmosphere
  • Drying Agents
  • pH Indicator Dyes
  • Approximate Figures for Concentrated Acids and Bases
  • Preservation of Museum Specimens
  • Collection, Preparation, and Mailing of Cultures and Specimens
  • Glassware Cleaning Solutions
  • Index

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